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Secretary of State for the Home Department. Md Sagor. Mediocurso — Estabelecimento de Ensino Particular Ld. Commission of the European Communities.

Mehmet Arslan v. Michael Schwarz v. Stadt Bochum. Mossa Ouhrami. Secretary of State for the Home Department and M. European Council.

Nold v. Oleificio Borelli SpA v. Ypourgos Esoterikon Kai Dioikitikis Anasygrotisis. Abdelaziz Arib. Pubblico Ministero v. Sadikou Gnandi v.

Skerdjan Celaj. Sophie Mukarubega v. Stauder v. City of Ulm. Storck KG v. Samir Zaizoune. Thi Ly Pham v.

Thyssen Stahl AG v. Council of the European Communities. Van Duyn v. Home Office. Van Gend En Loos v. Nederlandse Administratie Der Belastingen.

The Girls Yean and Bosico v. Dominican Republic. Republic of Angola. Regina v. Human Rights Committee hrc. Ahani v. Alzery v.

Arhuaco v. Baban v. Baboeram-Adhin et Al. Bakhtiyari v. Bautista de Arellana v. Byahuranga v. Danyal Shafiq v. Eshonov v. Fardon v.

Hamilton v. Herrera Rubio v. Husseini v. Judge v. Kalamiotis v. Kindler v. Kwok v. Madafferi v. Marcellana and Gumanoy v. Nystrom v.

Rajapakse v. Sri Lanka. Shams et Al. Suarez de Guerrero v. Winata v. Zhumbaeva v. Zundel v. Committee against Torture cat. Aemei v.

Agiza v. Alan v. Ayas v. Blanco Abad v. Dadar v. Dzemajl et Al. Elmi v. Halimi-Nedzibi v. Haydin v. Kisoki v.

Korban v. Kwami Mopongo et Al. Mutombo v. The Netherlands. Sonko v. Tala v. Tapia Paez v. International Law Commission ilc.

International Law Commission Special Rapporteur on the expulsion of aliens. Maurice Kamto, Special Rapporteur. General Assembly, July 5, UN General Assembly.

Expulsion of Aliens. New York Declaration for Refugees and Migrants. Concluding Observations: Austria.

Concluding Observations: Belgium. Concluding Observations: Canada. Concluding Observations: Cyprus. Concluding Observations: Czech Republic.

Concluding Observations: Finland. Concluding Observations: France. Concluding Observations: Greece.

Concluding Observations: Ireland. Concluding Observations: Israel. Concluding Observations: Italy. Concluding Observations: Lithuania.

Concluding Observations: Malta. Concluding Observations: Poland. Concluding Observations: Portugal. Concluding Observations: Serbia.

Concluding Observations: Spain. Concluding Observations: Switzerland. Concluding Observations: the United Kingdom. Concluding Observations: the United States of America.

General Comment No. Concluding Observations: Argentina. Concluding Observations: Australia. Concluding Observations: Denmark.

Concluding Observations: Germany. Concluding Observations: Iceland. Concluding Observations: Japan.

Concluding Observations: Kazakhstan. Concluding Observations: Kyrgyzstan. Concluding Observations: Liechtenstein.

Concluding Observations: Luxembourg. Concluding Observations: New Zealand. Concluding Observations: Norway. Concluding Observations: the Netherlands.

Concluding Observations: Turkey. Concluding Observations: Ukraine. February 9, General Recommendation No. Concluding Observations: Bulgaria.

Concluding Observations: Azerbaijan. Concluding Observations: China. Concluding Observations: Romania.

Concluding Observations: Russian Federation. Concluding Observations: Sweden. Concluding Observations: Albania. Concluding Observations: Andorra.

Concluding Observations: Ecuador. Concluding Observations: Fiji. Concluding Observations: Hungary. Concluding Observations: Jordan.

Concluding Observations: Korea. Concluding Observations: Malawi. Concluding Observations: Malaysia. Concluding Observations: Montenegro.

Concluding Observations: Singapore. Concluding Observations: Slovenia. Concluding Observations: South Africa. Concluding Observations: Armenia.

Joint General Comment No. Concluding Observations: European Union. Special Rapporteur on extrajudicial, summary or arbitrary executions.

Special Rapporteur on the Human Rights of Migrants srhrm. Special Rapporteur on the right to education.

Report of the Working Group on Arbitrary Detention. Conclusion on International Protection. Conclusion No.

Detention of Refugees and Asylum-Seekers. General Conclusion on International Protection. UN Doc. Note on International Protection.

Refugees Without an Asylum Country. Human Rights of Irregular Migrants. Regularisation Programmes for Irregular Migrants.

European Commission against Racism and Intolerance ecri. General Policy Recommendation No. May 10, Deportation of Foreign Nationals by Air.

Immigration Detention: Factsheet. European Committee of Social Rights. December 18, Advocate General. September 4, April 30, December 13, November 10, February 12, February 2, April 28, June 25, April 1, October 26, May 14, August 23, Council of the European Union.

June 9, June 5, November 9, Council Conclusions on the Future of the Return Policy. October 8, Return Action Programme. October 16, European Commission.

SEC , September 1, SEC , October 4, SEC , March 9, COM , July 19, COM , November 15, COM , October 14, COM , March 28, COM , November 30, COM , May 6, COM , September 9, COM 76, February 23, COM , March 2, COM , April 12, COM , November 18, COM , May 13, COM , June 7, Draft Return Handbook.

Annexed to C , COM , April 10, COM , July 13, COM , September 1, COM , September 12, COM , December 21, European Parliament. European Data Protection Supervisor.

May Report on Terrorism and Human Rights. Permanent Council of the Organization of American States.

Latin American Parliament parlatino. July 3, July 1, Andean Community. Andean Parliament. Central American Parliament. Pronunciamiento Del Parlamento Centroamericano.

July 4, South American Conference on Migration. Acosta , Diego , and Cian C. Murphy , eds. Modern Studies in European Law, Vol. Oxford : Hart Publishing , Aleinikoff , Thomas Alexander , and Vincent Chetail , eds.

Migration and International Legal Norms. The Hague : T. Asser Press , Pierre-Marie Dupuy. Leiden : Martinus Nijhoff Publishers , Amerasinghe , Chittharanjan Felix.

Principles of the Institutional Law of International Organizations. Cambridge Studies in International and Comparative Law. Cambridge : Cambridge University Press , Anderson , Bridget , Matthew J.

Gibney , and Emanuela Paoletti , eds. Immigrants and Minorities, Politics and Policy. New York : Springer , Oxford : Oxford University Press , Whose Freedom, Security and Justice?

Essays in European Law. Oxford : Hart , Balzacq , Thierry , and Sergio Carrera , eds. Security versus Freedom?

Aldershot : Ashgate , Bhabha , Jacqueline. Princeton: Princeton University Press , Boehm , Franziska. Berlin, Heidelberg : Springer , European Migration Law.

Ius Communitatis Series 3. Antwerp : Intersentia , Immigration and Asylum Law and Policy in Europe, vol.

Romano , and Ruth Mackenzie , eds. Ardsley NY : Transnational Publishers , Boswell , Christina , and Andrew Geddes.

Migration and Mobility in the European Union. The European Union Series. New York : Palgrave Macmillan , Brouwer , Evelien.

Immigration and Asylum Law and Policy in Europe, volume Bruxelles : Bruylant , Leiden : Brill Nijhoff , Ceccorulli , Michela , and Nicola Labanca , eds.

Routledge Advances in European Politics. Chetail , Vincent , ed. Research Handbook on International Law and Migration.

Cheltenham : Edward Elgar , Chetail , Vincent , and Vera Gowlland-Debbas , eds. The Graduate Institute of International Studies, vol.

The Hague [etc. Immigration and Asylum Law and Policy in Europe Clapham , Andrew. Collected Courses of the Academy of European Law, vol.

Coleman , Nils. Cornelisse , Galina. Costello , Cathryn. De Genova , Nicholas P. Delas , Olivier.

Mondialisation et Droit International Are Human Rights for Migrants? London : Routledge , Den Heijer , Maarten.

Europe and Extraterritorial Asylum. Studies in International Law. Desmond , Alan , ed. Pretoria : Pretoria University Law Press , Dijstelbloem , Huub , and Albert Meijer , eds.

Migration and the New Technological Borders of Europe. Basingstoke : Palgrave Macmillan , Dubin , Laurence , ed. Collection Droit Administratif — Administrative Law Eisele , Katharina.

Evans , Malcolm D. Flynn , Michael J. Flynn, eds. Fulchiron , Hughes , ed. Paris : L. J , Gammeltoft-Hansen , Thomas.

The Politics of International Migration Management. Migration, Minorities, and Citizenship. Goodwin-Gill , Guy S.

The Refugee in International Law. The Hague : Martinus Nijhoff Publishers , Gragl , Paul. Modern Studies in European Law. The Hague : Eleven International Publishing , Dordrecht : Springer , Guild , Elspeth , and Paul Minderhoud , eds.

Berlin Heidelberg : Springer , Hailbronner , Kay , ed. München : C. Beck , Hailbronner , Kay , and Daniel Thym , eds. Hamdan , Eman.

Nijmegen : Brill Nijhoff , Hathaway , James C. The Rights of Refugees under International Law. Henckaerts , Jean-Marie.

Hughes , Jane , and Fabrice Liebaut , eds. Oxford Monographs in International Law. Ineli-Ciger , Meltem.

Temporary Protection in Law and Practice. International Refugee Law Series, vol. Leiden; Boston : Brill Nijhoff , Juss , Satvinder Singh , ed.

Law and Migration. Farnham, Surrey : Ashgate , Paris : unesco , Kapuy , Klaus. Ius Commune Europaeum, vol.

Cambridge : Intersentia Publishers , Karanja , Stephen Kabera. Leiden; Boston : Martinus Nijhoff Publishers , Kindt , Els J.

Interestingly, distinct hierarchies of diabetogenic T-cell responses were also detectable in the NOD mouse model.

Prasad et al. This suggests that distinct CD8 T-cell responses, triggering the initial steps of beta cell destruction, play a prime role in the induction of diabetes [44].

At indicated times after immunization, blood glucose levels and cumulative diabetes incidences were determined.

We thank Katrin Schwengle and Andrea Wissmann for outstanding technical assistance. We thank Dr. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

National Center for Biotechnology Information , U. PLoS One. Published online Aug Bernhard O. Thomas WH. Kay, Editor. Author information Article notes Copyright and License information Disclaimer.

Competing Interests: The authors have declared that no competing interests exist. Received Apr 10; Accepted Jul 2. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

This article has been cited by other articles in PMC. Introduction Type 1 diabetes T1D is an autoimmune disorder, in which insulin-producing beta cells are destroyed by the cellular immune system [1] , [2] , [3].

Mice RIP-B7. Construction of Expression Plasmids The sequences of the different ppins antigens were codon-optimized and synthesized by GeneArt Regensburg, Germany.

Characterization of Antigen Expression Human embryonal kidney cells HEK cells were transiently transfected with the indicated plasmid DNAs using the calcium phosphate method.

Open in a separate window. Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. EPS Click here for additional data file.

TIF Click here for additional data file. References 1. Curr Opin Immunol 20 : — Immunity 32 : — Santamaria P The long and winding road to understanding and conquering type 1 diabetes.

Cold Spring Harb Perspect Med 2 : a Diabetes 56 : — J Clin Invest : — Annu Rev Immunol 23 : — J Exp Med : — F Biol Rep 2 : Nat Immunol 10 : — Diabetes 51 : — Eur J Immunol 37 : — J Immunol : — Diabetes 59 : — Nat Immunol 8 : — Annu Rev Immunol 26 : — J Exp Med : 63— Clin Immunol : 16— Tissue Antigens 62 : — Hum Genet : — Immunity 20 : — Int Immunol 10 : — Int Immunol 5 : — Nat Protoc 1 : 16— Immunogenetics 41 : — Curr Opin Immunol 15 : — Nature : — Nat Immunol 6 : — Biochem Soc Trans 36 : — Immunity 31 : — Curr Opin Immunol 24 : —

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Staatssecretaris van Veiligheid En Justitie. Khaled Boudjlida v. Kremzow v. Land Baden-Württemberg v. Panagiotis Tsakouridis.

Mary Carpenter v. Secretary of State for the Home Department. Md Sagor. Mediocurso — Estabelecimento de Ensino Particular Ld.

Commission of the European Communities. Mehmet Arslan v. Michael Schwarz v. Stadt Bochum. Mossa Ouhrami. Secretary of State for the Home Department and M.

European Council. Nold v. Oleificio Borelli SpA v. Ypourgos Esoterikon Kai Dioikitikis Anasygrotisis. Abdelaziz Arib.

Pubblico Ministero v. Sadikou Gnandi v. Skerdjan Celaj. Sophie Mukarubega v. Stauder v. City of Ulm. Storck KG v. Samir Zaizoune.

Thi Ly Pham v. Thyssen Stahl AG v. Council of the European Communities. Van Duyn v. Home Office. Van Gend En Loos v.

Nederlandse Administratie Der Belastingen. The Girls Yean and Bosico v. Dominican Republic. Republic of Angola. Regina v.

Human Rights Committee hrc. Ahani v. Alzery v. Arhuaco v. Baban v. Baboeram-Adhin et Al. Bakhtiyari v.

Bautista de Arellana v. Byahuranga v. Danyal Shafiq v. Eshonov v. Fardon v. Hamilton v. Herrera Rubio v.

Husseini v. Judge v. Kalamiotis v. Kindler v. Kwok v. Madafferi v. Marcellana and Gumanoy v. Nystrom v. Rajapakse v. Sri Lanka. Shams et Al.

Suarez de Guerrero v. Winata v. Zhumbaeva v. Zundel v. Committee against Torture cat. Aemei v. Agiza v. Alan v. Ayas v. Blanco Abad v.

Dadar v. Dzemajl et Al. Elmi v. Halimi-Nedzibi v. Haydin v. Kisoki v. Korban v. Kwami Mopongo et Al. Mutombo v. The Netherlands.

Sonko v. Tala v. Tapia Paez v. International Law Commission ilc. International Law Commission Special Rapporteur on the expulsion of aliens.

Maurice Kamto, Special Rapporteur. General Assembly, July 5, UN General Assembly. Expulsion of Aliens. New York Declaration for Refugees and Migrants.

Concluding Observations: Austria. Concluding Observations: Belgium. Concluding Observations: Canada.

Concluding Observations: Cyprus. Concluding Observations: Czech Republic. Concluding Observations: Finland. Concluding Observations: France.

Concluding Observations: Greece. Concluding Observations: Ireland. Concluding Observations: Israel. Concluding Observations: Italy. Concluding Observations: Lithuania.

Concluding Observations: Malta. Concluding Observations: Poland. Concluding Observations: Portugal. Concluding Observations: Serbia.

Concluding Observations: Spain. Concluding Observations: Switzerland. Concluding Observations: the United Kingdom.

Concluding Observations: the United States of America. General Comment No. Concluding Observations: Argentina.

Concluding Observations: Australia. Concluding Observations: Denmark. Concluding Observations: Germany.

Concluding Observations: Iceland. Concluding Observations: Japan. Concluding Observations: Kazakhstan. Concluding Observations: Kyrgyzstan.

Concluding Observations: Liechtenstein. Concluding Observations: Luxembourg. Concluding Observations: New Zealand.

Concluding Observations: Norway. Concluding Observations: the Netherlands. Concluding Observations: Turkey.

Concluding Observations: Ukraine. February 9, General Recommendation No. Concluding Observations: Bulgaria. Concluding Observations: Azerbaijan.

Concluding Observations: China. Concluding Observations: Romania. Concluding Observations: Russian Federation. Concluding Observations: Sweden.

Concluding Observations: Albania. Concluding Observations: Andorra. Concluding Observations: Ecuador.

Concluding Observations: Fiji. Concluding Observations: Hungary. Concluding Observations: Jordan.

Concluding Observations: Korea. Concluding Observations: Malawi. Concluding Observations: Malaysia. Concluding Observations: Montenegro.

Concluding Observations: Singapore. Concluding Observations: Slovenia. Concluding Observations: South Africa.

Concluding Observations: Armenia. Joint General Comment No. Concluding Observations: European Union.

Special Rapporteur on extrajudicial, summary or arbitrary executions. Special Rapporteur on the Human Rights of Migrants srhrm.

Special Rapporteur on the right to education. Report of the Working Group on Arbitrary Detention. Conclusion on International Protection.

Conclusion No. Detention of Refugees and Asylum-Seekers. General Conclusion on International Protection.

UN Doc. Note on International Protection. Refugees Without an Asylum Country. Human Rights of Irregular Migrants.

Regularisation Programmes for Irregular Migrants. European Commission against Racism and Intolerance ecri.

General Policy Recommendation No. May 10, Deportation of Foreign Nationals by Air. Immigration Detention: Factsheet.

European Committee of Social Rights. December 18, Advocate General. September 4, April 30, December 13, November 10, February 12, February 2, April 28, June 25, April 1, October 26, May 14, August 23, Council of the European Union.

June 9, June 5, November 9, Council Conclusions on the Future of the Return Policy. October 8, Return Action Programme.

October 16, European Commission. SEC , September 1, SEC , October 4, SEC , March 9, COM , July 19, COM , November 15, COM , October 14, COM , March 28, COM , November 30, COM , May 6, COM , September 9, COM 76, February 23, COM , March 2, COM , April 12, COM , November 18, COM , May 13, COM , June 7, Draft Return Handbook.

Annexed to C , COM , April 10, COM , July 13, COM , September 1, COM , September 12, COM , December 21, European Parliament.

European Data Protection Supervisor. May Report on Terrorism and Human Rights. Permanent Council of the Organization of American States.

Latin American Parliament parlatino. July 3, July 1, Andean Community. Andean Parliament. Central American Parliament.

Pronunciamiento Del Parlamento Centroamericano. July 4, South American Conference on Migration.

Acosta , Diego , and Cian C. Murphy , eds. Modern Studies in European Law, Vol. Oxford : Hart Publishing , Aleinikoff , Thomas Alexander , and Vincent Chetail , eds.

Migration and International Legal Norms. The Hague : T. Asser Press , Pierre-Marie Dupuy. Leiden : Martinus Nijhoff Publishers , Amerasinghe , Chittharanjan Felix.

Principles of the Institutional Law of International Organizations. Cambridge Studies in International and Comparative Law.

Cambridge : Cambridge University Press , Anderson , Bridget , Matthew J. Gibney , and Emanuela Paoletti , eds.

Immigrants and Minorities, Politics and Policy. New York : Springer , Oxford : Oxford University Press , Whose Freedom, Security and Justice?

Essays in European Law. Oxford : Hart , Balzacq , Thierry , and Sergio Carrera , eds. Security versus Freedom? Aldershot : Ashgate , Bhabha , Jacqueline.

Princeton: Princeton University Press , Boehm , Franziska. Berlin, Heidelberg : Springer , European Migration Law.

Ius Communitatis Series 3. Antwerp : Intersentia , Immigration and Asylum Law and Policy in Europe, vol.

Romano , and Ruth Mackenzie , eds. Ardsley NY : Transnational Publishers , Boswell , Christina , and Andrew Geddes.

Migration and Mobility in the European Union. The European Union Series. New York : Palgrave Macmillan , Brouwer , Evelien.

Immigration and Asylum Law and Policy in Europe, volume Bruxelles : Bruylant , Leiden : Brill Nijhoff , Ceccorulli , Michela , and Nicola Labanca , eds.

Routledge Advances in European Politics. Chetail , Vincent , ed. Research Handbook on International Law and Migration. Cheltenham : Edward Elgar , Chetail , Vincent , and Vera Gowlland-Debbas , eds.

The Graduate Institute of International Studies, vol. The Hague [etc. Immigration and Asylum Law and Policy in Europe Clapham , Andrew.

Collected Courses of the Academy of European Law, vol. Coleman , Nils. Cornelisse , Galina. Costello , Cathryn. De Genova , Nicholas P.

Delas , Olivier. Mondialisation et Droit International Are Human Rights for Migrants? London : Routledge , Den Heijer , Maarten. Europe and Extraterritorial Asylum.

Studies in International Law. Desmond , Alan , ed. Pretoria : Pretoria University Law Press , Dijstelbloem , Huub , and Albert Meijer , eds.

Migration and the New Technological Borders of Europe. Basingstoke : Palgrave Macmillan , Dubin , Laurence , ed.

Collection Droit Administratif — Administrative Law Eisele , Katharina. Evans , Malcolm D. Flynn , Michael J. Flynn, eds.

Fulchiron , Hughes , ed. Paris : L. J , Gammeltoft-Hansen , Thomas. The Politics of International Migration Management.

Migration, Minorities, and Citizenship. Goodwin-Gill , Guy S. The Refugee in International Law. The Hague : Martinus Nijhoff Publishers , Gragl , Paul.

Modern Studies in European Law. The Hague : Eleven International Publishing , Dordrecht : Springer , Guild , Elspeth , and Paul Minderhoud , eds.

Berlin Heidelberg : Springer , Hailbronner , Kay , ed. München : C. Beck , Hailbronner , Kay , and Daniel Thym , eds.

Hamdan , Eman. Nijmegen : Brill Nijhoff , Hathaway , James C. The Rights of Refugees under International Law. Henckaerts , Jean-Marie.

Pancreata were perfused in situ with collagenase P cat. Pancreatic cells were purified with Histopaque cat. To detect ppins-specific CD8 T-cell responses, we used a ppins-specific peptide library i.

Non-specific binding of antibodies to Fc-receptor was blocked by preincubating cells with mAb 2. Cells were washed and analyzed by FCM.

The statistical significance of diabetes induction in immunized mice was determined by the log-rank test. A Map of ppins antigens.

At indicated times after immunization, blood glucose levels B and cumulative diabetes incidences C were determined.

The statistical significance of diabetes induction in immunized mice was determined using the log-rank test.

Pancreatic cell preparations from ten mice were pooled and restimulated ex vivo for 16 hours with a ppins-specific peptide library i.

E RIP-B7. In group 4, the indicated plasmids were injected into the right and the left tibialis anterior muscles, respectively.

CD8 T-cells were prepared from pancreata of diabetic groups 2—4 or non-diabetic group 1 mice and restimulated ex vivo with A N21A or B 22—29 peptides.

These sequences contain an optimal K b -binding motif, i. Hence, after successful priming, the two CD8 T-cell populations do not interfere with each another.

During the course of EAD, the development of hyperglycemia correlated with an increasing influx of lymphocytes and CD8 T-cells into the pancreatic islets Figure 2C , groups 1—3.

B RIP-B7. Primary FACS data are shown for representative mice. At 28 h after transfection, cells were either non-treated lanes 1 and 2 , or incubated for 6 h with the proteasome-inhibitors expoxymycin ep; lane 3 or lactacystein lac; lane 4 and subsequently lysed.

In contrast, the expression of ppins in transiently transfected HEK cells was not changed by proteasome inhibitors [18]. Comparable with RIP-B7.

Pancreatic cell preparations from eight ppins-immune mice were pooled and restimulated ex vivo for 16 hours with a ppins-specific peptide library i.

The statistical significance of diabetes induction in immunized mice A—C was determined using the log-rank test. We next generated PD-L1-deficient mice, which selectively express the costimulatory B7.

Interestingly, immunization of RIP-B7. We next asked whether alternative B7. These events play a prominent role in the amplification of autoreactive immune responses and beta cell destruction [38] , [39] , [40].

Interestingly, a single manipulation of the PD-L1 mouse model i. It is thus unlikely that the initial CD8 T-cell priming phase i.

Expression of the tg B7. The interaction of B7. Interestingly, distinct hierarchies of diabetogenic T-cell responses were also detectable in the NOD mouse model.

Prasad et al. This suggests that distinct CD8 T-cell responses, triggering the initial steps of beta cell destruction, play a prime role in the induction of diabetes [44].

At indicated times after immunization, blood glucose levels and cumulative diabetes incidences were determined.

We thank Katrin Schwengle and Andrea Wissmann for outstanding technical assistance. We thank Dr. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

National Center for Biotechnology Information , U. PLoS One. Published online Aug Bernhard O. Thomas WH.

Kay, Editor. Author information Article notes Copyright and License information Disclaimer. Competing Interests: The authors have declared that no competing interests exist.

Received Apr 10; Accepted Jul 2. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

This article has been cited by other articles in PMC. Introduction Type 1 diabetes T1D is an autoimmune disorder, in which insulin-producing beta cells are destroyed by the cellular immune system [1] , [2] , [3].

Mice RIP-B7. Construction of Expression Plasmids The sequences of the different ppins antigens were codon-optimized and synthesized by GeneArt Regensburg, Germany.

Characterization of Antigen Expression Human embryonal kidney cells HEK cells were transiently transfected with the indicated plasmid DNAs using the calcium phosphate method.

Open in a separate window. Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. EPS Click here for additional data file. TIF Click here for additional data file.

References 1.

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